#Web 4399 com ddt portable
However, the major weaknesses of colorimetric or fluorescence analysis (single-mode detection approaches), and specifically EuNP-mediated FICT, are that personnel are required to operate the portable sensors, and that FICT shows weak signals when analyzing biological samples (such as blood, urine, or feces). Over the past few decades, europium nanoparticles (EuNPs) and colloidal gold nanoparticles (AuNPs) have been used in FICT and RDT, respectively, to detect infectious diseases. POCT includes two types of analysis: colorimetric (visual detection by rapid diagnostic test (RDT)) and fluorescence (ultra-violet light detection using portable medisensor by fluorescent immunochromatographic strip test (FICT)) analysis. The POCT approach can deliver results within ~15 to 20 min, and is thus the most preferable on-site detecting technique. Although such methods provide powerful approaches for the detection of infectious diseases, limitations include the complexity, high cost, essential training, suitable instruments, and high-quality samples required. Globally, numerous analytical devices have been used to detect infectious diseases, including enzyme-linked immunosorbent assay (ELISA), immunofluorescence, real-time reverse transcription-polymerase chain reaction (rRT-PCR), serological testing, the immunowall device, POCT, and nucleic acid sequencing. Overall, the developed Cys Au-EuNPs-mediated dual-mode POCT kit can be used as an effective nanocomposite for the development of on-site monitoring systems for infectious disease surveillance. The photo luminance (PL) stability of ~3% the Cys Au-EuNPs conjugates that was obtained under UV light irradiation differs considerably from that of the commercial nanoparticle conjugates. The obtained LOD is eight-fold that of commercial nanoparticle conjugates. The Cys Au-EuNP-mediated RDT (colorimetric analysis) and FICT kit revealed a limit of detection (LOD) of 10 HAU/mL and 2.5 HAU/mL, respectively, for H5N1 under different titer conditions. The particle size distribution revealed an average size of ~130 ± 0.66 nm for the Cys Au-EuNPs. The spectral characteristics, surface morphologies, functional groups, surface charge and stability of the Cys AuNPs, EuNPs, and Cys Au-EuNPs were confirmed by UV-visible spectrophotometry, fluorescence spectrometry, transmission electron microscope with Selected area electron diffraction (TEM-SAED), Fourier-transform infrared spectroscopy (FTIR) and zeta potential analysis. Commercial nanoparticles were used for comparison.
Herein, we prepare cysteamine-gold coated carboxylated europium chelated nanoparticle (Cys Au-EuNPs)-mediated POCT for the detection of the H5N1 avian influenza virus (AIV).
Sensitive and timely on-site POCT methods with high signal enhancement are therefore essential for the accurate diagnosis of infectious diseases. The testing kits are generally insufficient in terms of signal enhancement, which is a major drawback of this approach. Globally, point-of-care testing (POCT) is the most preferable on-site technique for disease detection and includes a rapid diagnostic test (RDT) and fluorescent immunochromatographic strip test (FICT).
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